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KMID : 0903519810240010015
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1981 Volume.24 No. 1 p.15 ~ p.20
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Effect of Foliar Spray of Urea on Urease Activity in various Plant Leaves
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홍종욱/Hong, Jong Uck
이효사/Lee, Hyo Sa
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Abstract
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Changes of unease activity in plant leaves following foliar application of urea were investigated with soybean, rye, tomato, radish and cabbage which were actively growing in a field. In this experiment, the procedure of the enzyme assay included incubation of the reaction mixture at 60℃ for 3 hr in order to inactivate heat unstable enzymes which may utilize ammonia produced by unease. The leaves with urea application showed somewhat higher unease specific activities for 2-4 days immediately after the foliar spray as compared with controls. The most difference of the specific activities between urea treatment and control was usually observed 2 days after urea application regardless of the plants. The difference of the specific activities disappeared completely 4 or 5 days following urea treatment. Protein contents in the leaves of soybean and tomato were increased for about. 5 days after urea treatment, while no significant difference was found with rye, radish and cabbage. Urea application showed slightly lower ammonia concentration. in the leaves which had higher unease activities. These results suggest that foliar spray of urea is very effective when nitrogen supply a required for rapid growth.
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